Emory Epithelial Pathobiology Research Development Center

拟议的“埃默里上皮病理生物学研究发展中心”将包括3个核心，以支持由9名资深研究人员组成的收集小组的研究计划，该小组拥有13笔NIH R01赠款，其中7笔由NIDDK资助，横跨埃默里大学医学院的4个系。此外，中心将支持5名初级成员的研究活动，这些成员被认为是新的调查人员。这组研究人员分享了一个中心研究主题，即了解消化道上皮的生物学和病理生物学。特别是，该中心支持的项目将集中于了解肠道上皮的生理、炎症和增殖反应。拟议的DDRDC应用程序的目标是为这群高度专注的研究人员提供共享资源和设施，以提高研究能力，促进新的研究方向，并促进研究人员之间的强有力的互动和合作。该中心建议的核心是：(A)基因表达分析核心，(B)图像分析核心，(C)细胞培养和单抗核心。核心A将包括一个具有生物信息学和生物统计学支持的专用基因微阵列设施，并将重点放在与消化系统上皮生物学和病理生物学相关的基因表达谱上。核心B将包括一个最先进的共焦显微镜和一个荧光激活的细胞分类器，以增强研究人员的图像分析能力。核心C将专注于为研究人员提供涉及极化上皮表型的实验的专门渗透培养支持(Transwell)，以及支持产生克隆抗体的设施。所有三个核心都将位于埃默里大学主校区新落成的怀特黑德生物医学研究大楼。大多数参与调查人员的实验室都设在同一大楼内，其余实验室都在很容易到达的距离内。预计在拟议核心设施的支持下，该中心将大大提高相关调查人员的研究能力，并帮助进一步促进在埃默里建立一个强大的上皮生物学和病理生物学小组，重点放在国家糖尿病、消化和肾脏疾病研究所(NIDDK)支持的特派团内的领域。

The proposed "Emory Epithelial Pathobiology Research Development Center" will include 3 cores to support the research programs by a collect group of 9 established investigators with a composite of 13 NIH R01 grants, 7 of which are funded by the NIDDK, and spans across 4 departments of the Emory University School of Medicine. In addition, the Center will support the research activities of 5 junior members who are considered new investigators. This group of investigators shares a central research theme of understanding the biology and pathobiology of the epithelium in the digestive tract. In particular, projects to be supported by the Center will concentrate on understanding the physiologic, inflammatory and proliferative responses of the gut epithelium. The GOAL of the proposed DDRDC application is to provide shared resources and facilities for this group of highly focused investigators in order to enhance research capability, promote new research directions, and foster strong interactions and collaborations among the researchers. The proposed cores of the Center are: (A) Gene Expression Analysis Core, (B) Image Analysis Core, and (C) Cell Culture and Monoclonal Antibody Core. Core A will include a dedicated cDNA microarray facility with bioinformatic and biostatistical support and will focus on the profiling of gene expression relevant to epithelial biology and pathobiology of the digestive system. Core B will include a state-of-the-art confocal microscope and a fluorescence activated cell sorter to enhance investigators' ability for image analysis. Core C will focus on providing investigators specialized permeable culture support (transwells) for experiments involving the polarized epithelial phenotype as well as a facility to support the generation of monoclonal antibodies. All three cores will be located in the newly completed Whitehead Biomedical Research Building on the main campus of the Emory University. The majority of participating investigators' laboratories are located in the same building and the remainders are located within easily accessible distance. It is anticipated that with the support of the proposed core facilities, the Center will greatly enhance the research capabilities of the involved investigators and help further foster the development of a strong epithelial biology and pathobiology group at Emory with a strong research focus on areas within the mission supported by the National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK).