ZEISS/31 STALLION DDI MULTI-DIMENSIONAL LIVE-CELL IMAGING WORKSTATION

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：约翰霍普金斯艺术与科学学院、工程学院、医学学院和公共卫生学院内的11名NIH资助的研究人员组成的多元化小组;以及马里兰州大学医学院的马里兰州大学生物技术研究所要求资金从Carl Zeiss/Intelligent Imaging Innovations购买Stallion DPI活细胞数字成像工作站。所要求的系统集成了Zeiss 200 M TIRF就绪平台，具有双超快速/高灵敏度Cascade 512 B EM数码相机; 405/488 FRAP/光活化激光模块;完全集成的31 Slidebook多维采集/分析软件;并且针对1）快速/超灵敏4D双通道采集; 2）去卷积; 3）FRET和比率成像; 4）颗粒跟踪进行了优化; 5）FRAP和光活化。新的最先进的工作站将补充我们大量使用的蔡司LSM 510 META和老化的Deltavision系统，同时在速度和灵敏度方面进行重大升级。重要的是，Stallion DPI将为霍普金斯的综合成像中心提供新的/独特的宽场荧光功能，该中心是霍姆伍德校园/霍普金斯范围内的显微镜资源，经常被多个学校和部门使用，包括超过60个实验室和约200个用户。我们的研究人员，都通过NIH的资金，工作在一个主机的基本细胞生物学，生物化学和生物分子相关的问题，包括，但不限于：端粒/着丝粒重组在肿瘤; HSV组装和成熟; MHC I运输和组装，膜组织和动力学;内体/溶酶体动力学;膜运输;多聚基因载体的运输;线粒体和细胞凋亡;唾液酸展示和细胞粘附;以及内吞作用机制。在所要求的配置中，Stallion DDI工作站将为我们不同的研究人员提供广泛的基本/高级活细胞显微镜功能，这些功能目前在霍普金斯霍姆伍德校园中是缺乏/不存在的。新系统将位于生物系的综合成像中心（NO：），并将纳入IIC现有的完善的再充电系统，以确保恢复供应和维护资金。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A diverse group of 11 NIH funded investigators, within the Johns Hopkins Schools of Arts and Sciences, Engineering, Medicine, and Public Health; and the University of Maryland Biotechnology Institute in the University of Maryland School of Medicine request funds to purchase a Stallion DPI Live-Cell digital Imaging workstation from Carl Zeiss/lntelligent Imaging Innovations. The requested system integrates a Zeiss 200M TIRF-ready platform with dual ultra-fast/highly sensitive Cascade 512B EM digital cameras; a 405/488 FRAP/photoactivation laser module; fully integrated 31 Slidebook multidimensional acquisition/analysis software; and is optimized for 1) rapid/ultra-sensitive 4D two channel acquisition; 2) deconvolution; 3) FRET and ratio imaging; 4) particle tracking; and 5) FRAP and photoactivation. The new state-of-the-art workstation will complement our heavily utilized Zeiss LSM 510 METAs and aging Deltavision system, while constituting a significant upgrade in speed and sensitivity. Importantly, the Stallion DPI will provide new/unique widefield fluorescence capabilities for the Hopkins' Integrated Imaging Center, a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >60 laboratories and ~200 users. Our investigators, all well funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: telomere/centromere reorganization in tumors; HSV assembly and maturation; MHC I trafficking and assembly, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; mitochondria and apoptosis; sialic acid display and cell adhesion; and mechanisms of endocytosis. In the requested configuration, the Stallion DDI workstation will afford our disparate group of investigators a broad range of basic/advanced live-cell microscopy capabilities that are presently deficient/absent from the Hopkins Homewood campus. The new system will be conveniently located, administered, and maintained, in the Biology Department's Integrated Imaging Center (NO: and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.