DEVELOPMENTAL NEUROTOXICOLOGY PROGRAM

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。2005年间，开发神经毒理学项目继续致力于正电子发射断层扫描(PET)程序的开发，以便与复杂反应时间行为测试结合使用。我们与灵长类中心PET成像服务核心主管Satoshi Minoshima博士合作，完成了对产前暴露于甲基汞的成年束猴进行功能性PET成像的培训程序。对反应时间测试范例和测试设备进行了更改，以适应功能成像的独特要求。已经实现了注射生理盐水的试运行。2005年成功地完成了六次PET会议。目前正在对这些PET会议的大脑图像进行审查。该实验室中的三个人，包括汤姆·伯巴赫博士，都获得了安全处理放射性材料的认证。Burbacher博士和Grant博士也在继续努力，将发育神经毒理学计划中使用的可操作性测试程序调整为用于年轻动物。为此，年龄从3个月到1.5岁的猕猴正在接受培训，让它们自愿进入测试椅，触摸并握住反应时间测试面板上的主杆(反应时间测试的必要先决条件)。我们使用的保持时间从0.5秒到5秒不等。虽然大多数动物可以成功地被训练成触摸家庭酒吧的短暂时间(长达3秒)，但更长的保持时间已被证明是更困难的。使用较短的触摸和保持时间，从一组动物中获得了稳定的反应时间和移动时间。反应时间的滴定将在2006年引入。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During 2005, the Developmental Neurotoxicology Program has continued concentrating on the development of the Positron Emission Tomography (PET) procedures to be used in conjunction with complex reaction time behavioral testing. In collaboration with Dr. Satoshi Minoshima, Director of the PET imaging service core at the Primate Center, we have completed training procedures for functional PET imaging in adult Macaca fascicularis prenatally exposed to methylmercury. Changes were made to the reaction time test paradigm and test equipment to accommodate the unique requirements of functional imaging. Pilot runs with saline injections have been achieved. Six successful PET sessions were accomplished in 2005. The brain images from these PET sessions are currently being reviewed. Three individuals in the laboratory, including Dr. Tom Burbacher, are certified in the safe handling of radioactive materials. There are also continued efforts by Drs. Burbacher and Grant to adapt the operant test procedures used in the Developmental Neurotoxicology Program for use with younger animals. To this end, macaque monkeys ranging from 3 months to 1.5 years are being trained to voluntarily enter the testing chair and touch and hold the home bar on the reaction time test panel (a necessary prerequisite for reaction time testing). We are using hold times that vary from .5 seconds to 5 seconds. While most animals can successfully be trained to touch the home bar for brief hold times (up to 3 seconds), longer hold times have proven to be more difficult. Using the shorter touch and hold times, stable reaction times and movement times have been obtained from a subset of animals. Titration of reaction times will be introduced in 2006.