ESTABLISHMENT OF A SPF RHESUS MACAQUE COLONY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。该项目的目标是提高国家生产无疱疹B病毒、SIV、SRV和STLV-1特异性病原体（SPF）的印度恒河猴（Macaca mulatta）的能力。拟议中的殖民地将继续生产高质量的遗传特征的动物用于艾滋病相关的研究，由美国国立卫生研究院赠款设在西南国家灵长类动物研究中心（SNPRC）和其他研究机构在美国西南地区。SNPRC殖民地的大部分原始种群是通过从美国空军（USAF）收购现有的SPF殖民地获得的。向SNPRC提供了一笔CO 6赠款（RR-01-130，SNPRC SPF恒河猴设施），用于建造新住房。拟定的生产和收购计划将增加殖民地规模，以达到430只动物的稳定状态。这一目标群体的大小将允许每年收获70只动物，每年开始在06年，以满足艾滋病相关的研究和美国空军的研究需求的关键需求。 已经并将继续对殖民地生产的SPF猴进行良好的血清学和遗传学表征。定期进行血清学检测并移除阳性动物，以确保SPF状态的维持。对SPF群体的所有成员进行15个微卫星标记的基因分型，其可用于评估母性和父性，并遗传表征殖民地。这些详细的遗传信息将被用于一个积极的遗传管理计划，旨在最大限度地减少近亲繁殖，最大限度地提高遗传变异性，并确保殖民地是完全纯种的。已经确定了每只动物的Mamu-A*01状态，并对部分殖民地采用了选择性育种策略，以产生携带该MHC等位基因的动物。将采用人口管理战略，通过制定最佳生产和收获时间表，确保殖民地的长期生存能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to increase the nation¿s capacity to produce Indian-origin rhesus monkeys (Macaca mulatta) that are specific pathogen-free (SPF) for herpes B virus, SIV, SRV, and STLV-1. The proposed colony will continue to produce high quality genetically characterized animals for use I AIDS-related research by NIH grantees based at the Southwest National Primate Research Center (SNPRC) and at other research institutions in the southwestern region of the United States. Most of the founding stock of the SNPRC colony was obtained through acquisition of an existing SPF colony from the U.S. Air Force (USAF) to the Primate Center. A CO6 grant (RR-01-130, SPF Rhesus Macaque Facility for SNPRC) was awarded to the SNPRC for the construction of new housing. The proposed production and acquisition schedule will result in increasing the colony size to achieve a steady state of 430 animals. This target colony size will allow an annual harvesting of 70 animals per year beginning in Year 06 to fulfill critical needs in AIDS-related research and research needs of the USAF. The SPF monkeys produced by the colony have been and will continue to be well characterized serologically and genetically. Serological testing and removal of positive animals is done on a routine basis to assure maintenance of SPF status. All members of the SPF colony are genotyped for 15 microsatellite markers, which can be used to assess maternity and paternity, and to genetically characterize the colony. This detailed genetic information will be used in an active genetic management program designed to minimize inbreeding, to maximize genetic variability, and to ensure that the colony is fully pedigreed. The Mamu-A*01 status of each animal has been determined, and a selective breeding strategy has been employed with a portion of the colony to produce animals which carry this MHC allele. Demographic management strategies will be used to ensure the long-term viability of the colony through development of optimal production and harvesting schedules.