VISUALIZING ASSOCIATION OF N-RAS IN LIPID MICRODOMAINS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。在巨大的单层囊泡上的双光子荧光显微镜用于跟踪荧光（Bodipy）标记和完全脂化（双十六烷基化）的N-Ras蛋白插入到异质脂质双层系统中。双层由典型的筏混合物POPC，鞘磷脂和胆固醇组成，其-取决于成分的浓度-分为液体无序（ld），液体有序（lo）和固体有序（So）。本实验的结果将为N-Ras在特定区域的优先分配提供直接证据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Two photon fluorescence microscopy on giant unilamellar vesicles is used to follow the insertion of a fluorescently(Bodipy)labelled and completely lipidated (bi-hexadecylated) N-Ras protein into heterogeneous lipid bilayer systems. The bilayer consists of the canonical raft mixture POPC, sphingomyelin and cholestero, which -depending on the concentration of the constituents-separates into liquid-disordered(ld), liquid-ordered(lo) and solid-ordered(So). The result of this experiment will provide the direct evidence of the preferable partitioning of N-Ras into specific domains.