ACTIVE STAINING FOR DEVELOPMENTAL TOXICOLOGY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。本项目旨在建立18-21日龄大鼠胎儿和21日龄大鼠幼鼠的活性染色和扫描方法，为今后的发育生物学和发育毒理学研究提供必要的基础。更具体地说，我们将做以下工作：开发灌注方法，将Gd造影剂（ProHance）带入标本以降低T1；利用定量T1/T2直方图滴定浓度，确定所选冠状切片弛豫时间的分布；开发3D采集协议，允许在每次扫描8小时内实现最高的信号噪声分辨率和器官清晰度；确定18-21天大鼠胎儿常规MR组织学所需的分辨率；确定21日龄幼鼠常规MR组织学所需分辨率；了解如何为MR组织学制定生物学相关的SOP。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to develop methods for active staining and scanning of 18-21 day rat fetuses and 21 day old rat pups to provide the necessary foundation for future studies in developmental biology and developmental toxicology. More specifically we will do the following: develop perfusion methods for carrying Gd contrast agent (ProHance) into the specimen to reduce T1; titrate the concentration using quantitative T1/T2 histograms to determine the distribution of relaxation times in selected coronal slices; develop a 3D acquisition protocol that will allow the highest possible signal to noise resolution, and organ definition in 8 hours per scan; determine the resolution required for routine MR histology in the 18-21 day rat fetus; determine the resolution required for routine MR histology of the 21 day rat pup; understand how to develop a biologically relevant SOP for MR histology.