PROTEINS UNDER PRESSURE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。柑橘碱是一种黄色荧光蛋白，在压力下会变色。它的发射光谱红移了约4 nm，当它被加压到3.5kbar时，荧光强度下降了约10%。2005年11月，我在F2使用高压冷冻晶体进行了实验。我观察到发光元件发色团的逐渐变形，简单的量子力学模型表明，这是红移的原因。我想将我的高压测量扩展到包括室温晶体，以查看使用铍高压X射线结晶盒在室温下是否发生同样的变形。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Citrine is a yellow fluorescent protein that changes color under pressure. Its emission spectrum redshifts by around 4 nm, and the intensity of the fluorescence drops by around 10% when it is pressurized to 3.5 kBar In November of 2005 I performed experiments at F2 using high pressure frozen crystals. I observed a progressive deformation of the light emitting element, the chromophore, which simple quantum mechanical modelling indicates is the cause of the red shift. I would like to extend my high pressure measurements to include room temperature crystals, to see if the same deformation occurs at room temperature using a beryllium high pressure X-ray crystallographic cell.