CITRINE UNDER VARIABLE LIGHT CONDITIONS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。柑橘碱是一种黄色荧光蛋白，在压力下会变色。它的发射光谱红移了大约4 nm，当它被加压到3.5kbar时，荧光强度下降了大约10%。在以前的国际象棋实验中，在2005年11月，我观察到发光元件发色团的逐渐变形，简单的量子力学模型表明这是红移的原因。伊利诺伊大学香槟分校马丁内斯小组对发色团形变的更详细的量子模型表明，这种分子的激发态和基态可能通过构象变化来区分。简单地说，当分子吸收一个光子时，它就会改变它的构象。我相信在我的X射线数据中可能有这种构象转换的证据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Citrine is a yellow fluorescent protein that changes color under pressure. Its emission spectrum redshifts by around 4 nm, and the intensity of the fluorescence drops by around 10% when it is pressurized to 3.5 kBar In previous CHESS experiments, in November of 2005, I have observed a progressive deformation of the light emitting element, the chromophore, which simple quantum mechanical modelling indicates is the cause of the red shift. More detailed quantum modelling of the chromophore`s deformation by the Martinez group at the University of Illinois at Urbana-Champaign suggests that the excited and ground states of this molecule may be distinguished by a conformational change. Simply put, when the molecule absorbs a photon of light, it changes its conformation. I believe that there may be evidence for this conformational switch in my X-Ray data.