NMR STUDY OF THE TITRATION OF YGGX WITH GALLIUM III

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。YggX是一种11 kDa的蛋白质，在肠沙门氏菌以及其他几种不同的细菌中发现，它与铁的运输和保护细胞免受氧化损伤有关。对纯化的YggX的圆二色谱研究表明，该蛋白在铁III和镓III作用下发生了构象变化。为了了解这种构象变化，需要更详细的结构数据。N15标记的YggX蛋白样本将与镓III样本滴定，以获得详细的结构数据。然后将使用该实验的结果来确定应该使用什么形式的YggX来确定溶液结构

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. YggX is an 11 kDa protein found in Salmonella enterica as well as in several other diverse bacteria that has been implicated in iron trafficking and in protecting the cell from oxidative damage. Circular dichroism studies on purified YggX have shown that this protein undergoes a conformational change upon exposure to iron III as well as to gallium III. In order to understand this conformational change in more detailed structural data is needed. An N15 labeled sample of YggX protein will be titrated with a sample of gallium III in order to obtain detailed structural data. The results of this experiment will then be used in order to decide what forms of YggX should be used for determining a solution structure