CORE RESEARCH PROJECT 2: CRYO-TILT STAGES FOR TOMOGRAPHY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。除了显微镜本身之外，最关键的仪器是对生物样品进行成像的载物台。该装置必须在成像期间实现低温稳定样品的精确计算机控制的平移和旋转。载物台还必须与用于快速冷冻样品的仪器连接，并允许以简单的密封方式将冷冻样品转移到X射线显微镜。这将需要两个单独的阶段：一个用于圆柱形样品，第二阶段用于安装在平面基板上的平面样品。我们首先将注意力集中在圆柱形样品的载物台上，因为已经构建了一个非常有效的原理验证原型载物台。然后我们将开始开发平板样品冷冻台。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The most critical instruments to be constructed, other than the microscope itself, are the stages for imaging biological samples. The devices must achieve precise computer controlled translation and rotation of the cryogenically stabilized sample during imaging. The stages must also interface with instrumentation used to rapidly freeze the samples and allow transfer of the frozen sample to the x-ray microscope in a simple hermetic fashion. This will require two separate stages: one for cylindrical samples and a second stage for flat samples mounted on planar substrates. We will focus our attention on the stage for cylindrical samples first, since a proof-of-principle prototype stage that functions quite efficiently has been constructed. We will then begin development of the flat specimen cryostage.