EFFECTS OF ULTRAVIOLET IRRADIATION ON VITAMIN D AND CALCIUM

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项临床研究方案的主要目标是确定不同皮肤类型的成年人在多次亚温暴露于模拟阳光下时循环中维生素D及其代谢物浓度的定量变化。患者将在秋末和冬季暴露在日光浴床上三个月。据推测，在维生素D缺乏的受试者中，暴露于日光浴床辐射会增加血清维生素D和25(OH)D水平，降低血清甲状旁腺素和尿NTX水平。这项临床研究方案的第二个目标是从穿孔活检中定量测量与维生素D新陈代谢、细胞增殖和分化、DNA损伤和修复、B-内啡肽和昼夜节律相关的基因的表达。此外，我们将在研究期间使用Datacolor微型闪光分光光度计在每次照射之前和之后立即测量皮肤色素沉着，以量化黑色素色素沉着的程度和因模拟日光照射而导致的任何红斑

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary goal of this clinical research protocol is to determine, in adults with varying skin types, the quantitative changes in circulating concentrations of vitamin D and its metabolites in response to multiple suberythemal exposures to simulated sunlight. Patients will be exposed to a tanning bed for three months during late fall and winter. It is hypothesized that exposure to tanning bed radiation will increase serum vitamin D and 25(OH)D levels, and reduce serum PTH and urine NTX in subjects who are vitamin D insufficient. The secondary goal of this clinical research protocol is to quantitatively measure from punch biopsies, the expression of genes related to vitamin D metabolism, cell proliferation and differentiation, DNA damage and repair, B-endorphin and circadian rhythm. In addition, we will measure skin pigmentation before and immediately after each irradiation during the study using Datacolor microflash spectrophotometer to quantitate the degree of melanin pigmentation and any erythema that results from exposures to simulated sunlight in a tanning be