AMBIENT PARTICULATE MATTER AND HUMAN BRONCHIAL EPITHELIAL CELLS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。本研究将对颗粒物（PM）组分对HBEC合成GM-CSF的影响进行一些生化和细胞实验，例如鉴定参与GM-CSF表达的MAPK/Raf途径。还将评价细胞因子（IL 4、5、13和TNF-α）在增加GM-CSF释放中的作用。将研究PM的特定化学性质在触发GM-CSF中的可能参与。由于GM-CSF参与树突状细胞的成熟，该研究还将探讨PM是否会导致肺树突状细胞的变化。本研究已在GCRC上启动，受试者继续入组。结果尚未公布。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study will conduct a number of biochemical and cellular experiments on the effect of particulate matter (PM) fractions on GM-CSF synthesis by HBEC, such as the identification of MAPK/Raf pathways involved in GM-CSF expression. The effects of cytokines (IL4, 5, 13 and TNF-alpha) in increasing the release of GM-CSF also will be evaluated. The possible involvement of specific chemical properties of the PM in triggering GM-CSF will be studied. Since GM-CSF is involved in dendritic cell maturation, the study will also address whether PM causes changes in lung dendritic cells. This study has been initiated on the GCRC, and subjects continue to be enrolled. Results are not yet available.