AMINO ACID SUPPLEMENTATION AND MUSCLE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们之前对年轻人的研究表明，最小阻力运动和补充必需氨基酸的营养可以维持瘦体重（LBM），并保持或减少肌肉功能的丧失。因此，该项目的目标是研究必需氨基酸单独或与阻力或步行运动相结合对老年人肌肉蛋白质代谢，LBM和肌肉功能的影响。 这项研究的目的是确定老年人的肌肉和激素系统对10天卧床休息的反应。该研究还将确定氨基酸（蛋白质）补充和运动是否可以减少肌肉损失并在10天卧床休息期间保持肌肉功能。 我们建议研究三组老年男女（65-80岁）在卧床休息10天之前、期间和之后：1）每天接受三次EAA补充剂（EAA），2）在卧床休息期间接受相同的EAA补充剂加三次阻力运动（EAA+RE），3）接受EAA补充剂加标准护理每日步行运动（EAA+WE）。我们将研究的假设，EAA和这些运动方案的组合将更有效地保持肌肉蛋白质合成，LBM和肌肉功能比单独干预。此外，我们建议这些干预措施的结合将加快从卧床休息中恢复。最后，我们建议这些干预措施或干预措施的组合将有效地维持老年人的肌肉蛋白质合成，肌肉质量和功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our previous investigations in younger individuals demonstrated that minimal resistance exercise and nutritional supplementation with essential amino acids can maintain lean body mass (LBM) and preserve or diminish the loss of muscle function. Therefore, the goal of this project is to examine the effects of essential amino acids alone or in combination with resistance or walking exercise on muscle protein metabolism, LBM and muscle function in older individuals. The purpose of the study is to determine how the elderly's muscle and hormonal system respond to 10 days of bedrest. The study will also determine if amino acid (protein) supplementation and exercise can reduce muscle loss and maintain muscle function during 10 days of bedrest. We propose to study three groups of older men and women (65-80 years) before, during, and after 10 days of bedrest: 1) will receive three supplements per day of EAA alone (EAA), 2) will receive the same EAA supplementation plus three sessions of resistance exercise throughout bedrest (EAA+RE), and 3) will receive EAA supplementation plus the standard-of-care daily walking exercise (EAA+WE). We will examine the hypotheses that the combination of EAA and these exercise regimens will be more effective in preserving muscle protein synthesis, LBM, and muscle function than either intervention alone. Further, we propose that combination of these interventions will expedite recovery from bedrest. Finally, we propose that these interventions, or combination of interventions, will be effective in maintaining muscle protein synthesis, muscle mass, and function in the elderly.