NE COBRE: PROTEOMICS AND GENOMICS CORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。该核心的作用是在分子的测量和表征领域为病毒学中心的成员提供支持。微阵列服务通过这一核心向三个不同设施的国家志愿人员组织成员提供，一个在联合国后勤基地，两个在联合国军事委员会。在UNL校园，质谱组件集中精力，特别是在蛋白质组学领域。 蛋白质组学涉及使用质谱鉴定蛋白质并确定在细胞生物学中对这些蛋白质进行的修饰。在我们的实验室中，我们将联合收割机湿化学处理方法与色谱法、先进的质谱技术和使用数据库挖掘的计算方法相结合，以鉴定可能参与与病毒相关的各种生物过程的蛋白质。使用一维SDS-PAGE和二维IEF-SDS-PAGE进行这些研究，然后进行胶内胰蛋白酶消化，并用反相HPLC结合电喷雾-四极杆-飞行时间质谱法分离胰蛋白酶片段。根据已知蛋白质的专有和公共领域数据库，对这些LC/MS和LC/MS/MS实验的结果进行内部检索。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The role of this core is to provide support to members of the Virology Center in the areas of measurement and characterization of molecules. Micro array services are provided through this core to NCV members at three different facilities with one at UNL and two at UNMC. On the UNL campus, the mass spectrometry component concentrates its efforts specifically on the area of proteomics. Proteomics involves using mass spectrometry for the identification of proteins and in determining modifications that are made to these proteins in the biology of the cell. In our laboratory we combine wet chemical processing methods with chromatography, advanced mass spectrometry techniques and computing methods using database mining to identify proteins which may be involved in the various biological processes associated with viruses. These studies were performed using both one dimensional SDS-PAGE and 2-dimensional IEF-SDS-PAGE, followed by in-gel trypsin digestion and separation of tryptic fragments with reverse phase HPLC coupled with electrospray -quadrapole-time-of-flight mass spectrometry. The results from these LC/MS and LC/MS/MS experiments were searched in-house against both proprietary and public domain databases of known proteins.