COBRE: OK MED RES FOUND: CORE I: IN VITRO MICROSCOPY CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。先进的显微镜技术代表了我们多学科计划的每个拟议项目的主要研究方法。选择最合适的方法可能是非常复杂的，并且样品制备中涉及的技术需要相当的技能。因此，体外显微镜核心将在这个合作项目中发挥特别重要的作用。该中心将为项目的各个步骤提供最先进的专业知识，从实验设计和标本制备，到高分辨率光学和电子显微照片的图像捕获和分析。体外显微镜核心将设在最近成立的心血管生物学研究项目成像设施，由弗洛里亚·卢普博士指导。Andrew Westmuckett博士是一位经验丰富的成像专家，他将把50%的精力投入到本提案中描述的活动中。核心拥有专业知识和设备来执行各种技术，从明场组织学分析到共聚焦显微镜和3D成像，从标准透射电子显微镜到免疫金标记和先进的冷冻技术（冷冻免疫金标记，冷冻替代，高压冷冻，冷冻断裂和深度蚀刻）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Advanced microscopy techniques represent major investigational approaches in each of the proposed projects of our multi-disciplinary program. The choice of the most appropriate method can be very complex, and the techniques involved in sample preparation require considerable skill. Therefore the In Vitro Microscopy Core will play a particularly important role in this collaborative program. The Core will provide state-of-the-art expertise in various steps of the projects, from experimental design and specimen preparation, to image capture and analysis of high-resolution light and electron micrographs. The In Vitro Microscopy Core will be based in the recently established Imaging Facility of the Cardiovascular Biology Research Program, under the direction of Dr. Florea Lupu. An experienced imaging specialist, Dr. Andrew Westmuckett will devote 50% of his effort towards the activities described in this proposal. The core has the expertise and the equipment to perform a diversity of techniques, ranging from brightfield histological analysis to confocal microscopy and 3D imaging, from standard transmission electron microscopy to immunogold labeling and advanced cryo-technologies (cryoimmunogold labeling, freeze substitution, high pressure freezing, and freeze fracture and deep-etching).