AUTOMATED FINDING OF MODIFIED PEPTIDES FROM TANDEM MS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。通过PTM或通过氨基酸突变进行肽修饰对许多领域的研究人员都很重要。磷酸化信号，氧化蛋白质损伤的例子。这些修饰影响蛋白质结构和反应性，如果人工诱导，可以用于研究蛋白质结构。在许多情况下，修饰的确切性质是不知道的，或者有太多的修饰需要考虑使用蛋白质数据库搜索程序。编写了一个通用程序来查找任何类型的修改。它是基于2D相关的串联MS的理论光谱或现有的光谱的未修饰的肽。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Peptide modification by PTM or by mutation of amino acids is important to researcher in many fields. Phosphorilation for signaling, oxidation in protein damage are examples. These modifications affect protein structure and reactivity and can be used to study protein structures if induced artificially. In many cases the exact nature of the modification is not known or there are too many of them to consider using a protein database search program. A general program to look for any kind of modification was written. It is based on 2D correlation of tandem MS to theoretical spectra or to existing spectra of unmodified peptides.