Core B: Imaging/Marker Core

PPG的核心B为所有项目的标准化细胞染色方法提供了重要支持， 细胞计数和共聚焦显微镜，协调体内动物成像，开发新的成像方法， 和新的蛋白质“足迹”质谱法。新的强大的蛋白质足迹 增加到核心B服务的技术提供了有关蛋白质-核酸和蛋白质-蛋白质的详细信息 交互.成像蛋白质核心将提供具有质量保证的公共资源， PPG中的五个项目。多用户仪器，包括Leica共聚焦显微镜和MS， 活体成像系统由经过培训的技术人员以具有成本效益的方式使用。共焦成像是一种 PPG中几个关键项目的基本分析形式。使用MS的活体动物成像 仪器正迅速成为体内纵向Veal时间分析肿瘤的标准 分布和病毒的表达，是必不可少的几个建议的工作，在竞争性更新。 AXIMA-CFR（MALDI-TOF）质谱仪是一种测量蛋白质-蛋白质、蛋白质 核酸和蛋白质抑制剂，并已被添加为核心B服务。核心B在一个突出的 环境，并记录了与所有项目的互动，包括许多科学出版物。 成像蛋白质核心B的具体目的包括：1）提供标准化的细胞染色服务 将通过PPG成像核心的共聚焦成像或流式细胞术分析的样本 通过综合癌症中心的细胞分析实验室共享服务，2） 进行标准化研究，利用共聚焦显微镜成像进行定量分析， 病毒和细胞蛋白分布和亚细胞定位。为这些人提供培训和其他技术， PPG用户组，3）协调对MS成像系统的访问，并为PPG用户提供培训 4）开发成像仪器的新应用，以增强PPG定向 研究并引导更精细的图像分析，5）采用质谱法（AXIMA-CFR MALDITOF） 基于蛋白质足迹技术的蛋白质-核酸和蛋白质蛋白质的详细表征 交互.

Core B of the PPG provides important support for all projects in standardized cell staining methods for flow cytometry and confocal microscopy, coordinates in vivo animal imaging, develops new imaging methods, and novel protein "foot printing" mass spectrometry methods. The new powerful protein foot-printing technology added to Core B services provides detailed information on protein-nucleic acid and proteinprotein interactions. The Imaging-Protein Core will provide common resources with quality assurance to the Five projects in the PPG. Multiuser instrumentation including the Leica Confocal Microscope and the MS in vivo Imaging System are used in a cost effective manner by trained technicians. Confocal imaging is an essential form of analysis for several key projects in the PPG. Live animal imaging using the MS instrumentation is rapidly becoming the standard for in vivo longitudinal Veal time' analysis of tumor distribution and virus expression and is essential for several of the proposed work in the competitive renewal. The AXIMA-CFR (MALDI-TOF) mass spectrometry is a key instrument to measure protein-protein, protein nucleic acid and protein-inhibitor, and has been added as a Core B service. Core B is within an outstanding environment and has documented interactions with all projects including numerous scientific publications. The Specific Aims of the Imaging-Protein Core B include: 1) To provide a standardized cell staining service of samples that will be analyzed by either confocal imaging through the PPG Imaging Core, or flow cytometry through the Analytical Cytometry Laboratory Shared Service of the Comprehensive Cancer Center, 2) To conduct standardized studies that makes use of Confocal Microscopy imaging for the quantitative analysis of virus and cell protein distribution and subcellular localization. To provide training and other techniques to the PPG user group, 3) To coordinate access to the MS Imaging System and provide training to the PPG user group, 4) To develop novel applications of the imaging instrumentation that will enhance the PPG directed research and lead more refined image analysis, 5) To employ the mass spectrometry (AXIMA-CFR MALDITOF) based protein foot printing technology for detailed characterization of protein-nucleic acid and proteinprotein interactions.