Regulation of Energy Metabolism in Insects

到目前为止用Ae.埃及人代表了第一个动态的方法来决定 蚊子的食物蛋白质中的氨基酸。使用在上一次资助期间制定的方法，我们 现在能够更详细地探索氨基酸代谢的代谢控制，并开始 分析幼虫发育过程中代谢燃料的积累，以及将这些方法应用于其他 蚊子，如淡色库蚊。这个项目的最终目标是定义血粉蛋白质氨基酸。 在整个蚊子的新陈代谢背景下，并从数量上理解如何不同 代谢流同时支持血餐后的性腺营养循环和 雌性蚊子，因为除非她在第一个性腺营养周期中幸存下来，否则就没有机会 吃第二顿血会传播疾病。这样的分析提供了一个独特的机会来识别 和意想不到的蚊子控制目标。 为下一批赠款提出了以下具体目标： 目的1研究Ae血粉氨基酸的代谢命运。埃及伊蚊雌性。 蚊子是如何有选择地改变不同氨基酸的代谢命运的？ 血中主要的血淋巴氨基酸--脯氨酸在血餐中的代谢命运是什么 消化？ 氨基酸氧化释放的氨代谢是如何变化的？ 性腺营养循环？ 目的2建立准确描述Ae代谢通量的定量模型。埃及伊蚊雌性。 ？哪种模型最好地描述了生殖营养周期中氨基酸代谢的时间进程？ 如何应用类似的定量模型来理解蚊子的整个新陈代谢？ 目的3研究胚胎发育过程中代谢流的动态变化。埃及伊蚊。 幼虫的新陈代谢如何整合，以确保能量储备的合成？ 目的4确定在特定组织中发生的代谢变化。埃及伊蚊雌性。 在不同的代谢条件下，蛋白质周转的程度如何？ 在性腺营养周期中，脂肪体和中肠的新陈代谢是如何变化的？ 目的研究淡色库蚊滞育过程中代谢流的动态变化。 滞育如何改变淡色库蚊的糖代谢？ ·淡色库蚊滞育期间储藏物的命运如何？

The results obtained so far with Ae. aegypti represent the first dynamic approach to determining the fate of amino acids from meal proteins in mosquitoes. Using the methods developed during the last grant period, we are now in a position to probe the metabolic control of amino acid metabolism in more detail and to begin an analysis of metabolic fuel accretion during larval development, as well as, applying these methods to other mosquitoes, such as Culex pipens. The ultimate aim of this project is to define blood meal protein amino acid metabolism in the context of the whole mosquito and to understand, in quantitative terms, how the different metabolic fluxes simultaneously support the gonotrophic cycle after a blood meal and the survival of the female mosquito, because unless she survives the first gonotrophic cycle there would be no opportunity to take a second blood meal and transmit disease. Such an analysis offers a unique opportunity to identify new and unexpected targets for mosquito control. The following specific aims are proposed for the next grant period: Aim 1 Investigate the metabolic fates of blood meal amino acids in Ae. aegypti females. ¿ How is the mosquito able to selectively alter the metabolic fate of different amino acids? ¿ What are the metabolic fates of proline, the major hemolymph amino acid, during blood meal digestion? ¿ How does the metabolism of ammonia released from amino acid oxidation change during a gonotrophic cycle? Aim 2 Develop quantitative models that accurately describe metabolic flux in Ae. aegypti females. ¿ What model best describes the time course for amino acid metabolism during the gonotrophic cycle? ¿ Howcan similar quantitative models be applied to understanding whole mosquito metabolism? Aim 3 Investigate the dynamics of metabolic flux during development in Ae. aegypti. ¿ How is the metabolism of larvae integrated to ensure the synthesis of energy reserves? Aim 4 Determine metabolic changes that occur in specific tissues ofAe. aegypti females. ¿ What is the extent of protein turnover under different metabolic conditions? ¿ Howdoes the metabolism of the fat body and midgut change during the gonotrophic cycle? Aim 5 Investigate the dynamics of metabolic flux during diapause in C.pipiens. ¿ Howdoes diapause alter sugar metabolism in C.pipiens? ¿ What is the fate of reserves during diapause in C.pipiens?