EFFECT OF CYTOKINES IN HOST DEFENSE AND INFLAMMATION

中性粒细胞在感染和炎症部位的积聚是 对宿主防御至关重要 当太少的中性粒细胞聚集在那里时 是增加了感染的易感性 当过多的中性粒细胞 积累会造成组织破坏。 近年来 细胞因子是多种细胞的产物， 吸引中性粒细胞炎症。 这些细胞因子之一，IL-8是 对于动员正常数量的嗜中性粒细胞到炎性细胞至关重要。 网站.在过去的一年里，我们对IL-8的合成进行了研究， 中性粒细胞的强效化学引诱剂。 以前我们 表明当人血中性粒细胞迁移到组织中时， 它们被诱导产生大量的IL-8。 在97财政年度 我们利用人类受试者外周血正常中性粒细胞， 我们研究了钙代谢和IL-8的合成和释放。 我们 研究表明，中性粒细胞IL-8的合成有两种途径，一种是 钙依赖型和非钙依赖型。

Accumulation of neutrophils to sites of infection and inflammation is essential to host defense. When too few neutrophils accumulate there is increased susceptibility to infection. When too many neutrophils accumulate there can be tissue destruction. In recent years cytokines, products of numerous types of cells, have been shown to attract neutrophils to inflammation. One of these cytokines, IL-8 is critical for mobilizing normal numbers of neutrophils to inflammatory sites. This past year our investigations of the synthesis of IL-8, a potent chemoattractant, by neutrophils were continued. Previously we demonstrated that when human blood neutrophil s migrate into tissues they are induced to make abundant amounts of IL-8. In fiscal year '97 we utilized peripheral blood normal neutrophils from human subjects and we studied calcium metabolism and IL-8 synthesis and release. Our studies demonstrated two pathways for neutrophil IL-8 synthesis, one calcium dependent and the other calcium independent.