MoFlow High Speed Fluorescence Activated Cell Sorter

描述(申请人提供)：这是一个修订的申请资金的达科细胞分裂MoFlo高速荧光激活细胞分选机。阿尔伯特·爱因斯坦医学院(AECOM)目前只有一台高速细胞分选机可供大多数研究人员使用，不足以满足当前的需求。申请的资金将使我们能够在我们的主要FACS核心设施中增加第二台高速分选机，这将使细胞分选能力翻一番，并扩大可支持的应用范围。该设施为大约275名用户提供了大多数基于FACS的资源，这些用户与AECOM由NIH资助的50多名调查人员的实验室有关。该机制拥有高度演变的业务计划、健全的财务监督和长期的强有力的机构支持。MoFlo分拣机有两名具有广泛操作背景的全职技术人员，不久将聘请第三名具有类似技能的人。有一位教员导师(本SIG应用程序的PI史蒂文·A·波塞利博士)是微生物学和免疫学教授，也是国际知名的研究免疫学家。由七名杰出的AECOM教员组成的指导委员会定期开会，为当前的运营和未来的增长制定政策，并提供质量保证。我们为其申请资金的仪器将有三个激光器，以激发各种不同的荧光探针。通过同时测量两个光散射参数和最多七个荧光通道，悬浮液中的细胞和亚细胞结构可以以超过每秒25,000个事件的速度进行表征和分类。该仪器可以从复杂的混合细胞悬液中分离特定类型的细胞，是基于特定荧光探针或抗体结合而纯化细胞和某些亚细胞结构的最有效和最精确的方法。所要求的仪器将被安置在生物安全二级(BSL-2)实验室空间，并将配备气雾剂密封舱，从而使我们能够根据我们的机构生物安全委员会对含有需要BSL-2预防措施的传染病的样本进行高速分选。提供资金支持购买这一仪器将加快研究癌症、糖尿病、心血管和传染病等重大公共卫生问题的原因和治疗方法。收购该仪器还将在促进AECOM作为先进生物医学研究和教育的主要中心的持续发展方面发挥重要作用。

DESCRIPTION (provided by applicant): This is a revised application for funding of a DakoCytomation MoFlo high speed fluorescence activated cell sorter. There is currently only one high speed cell sorter available to most investigators at the Albert Einstein College of Medicine (AECOM), which is inadequate to meet current needs. The requested funding will enable us to add a second high speed sorter to our main FACS Core Facility, which will double cell sorting capacity and extend the range of applications that can be supported. The Facility provides most FACS-based resources for approximately 275 users associated with the laboratories of more than 50 NIH- funded investigators at AECOM. The Facility has a highly evolved plan of operations, sound financial oversight, and a long history of strong institutional support. There are two full-time technical staff with extensive background in operation of the MoFlo sorter, and a third person with comparable skills will soon be hired. There is a faculty supervisor (Dr. Steven A. Porcelli, PI of this SIG application) who is a Professor of Microbiology and Immunology and an internationally known research immunologist. A steering committee composed of seven distinguished AECOM faculty meets regularly to set policy for current operations and future growth, and provide quality assurance. The instrument for which we have requested funding will have three lasers to enable excitation of a broad range of different fluorescent probes. Cells and subcellular structures in suspension can be characterized and sorted at speeds exceeding 25,000 events per second using simultaneous measurement of two light scatter parameters and up to seven channels of fluorescence. This instrument permits the isolation of specific cell types from among complex mixed cell suspensions, and is the most powerful and precise method available for purification of cells and certain subcellular structures based on the binding of specific fluorescent probes or antibodies. The requested instrument will be housed in biosafety level-2 (BSL-2) laboratory space and will be equipped with an aerosol containment module, thus enabling us to perform high speed sorting of samples containing infectious agents requiring BSL-2 precautions in accordance with our Institutional Biosafety Committee. The provision of funds to support the purchase of this instrument will accelerate research into the causes and treatment of major public health problems including cancer, diabetes, cardiovascular and infectious diseases. The acquisition of this instrument will also play a significant role in facilitating the continuing growth of AECOM as a major center for advanced biomedical research and education.