ANTIBIOTIC RESISTANCE MUTATIONS IN H MARISMORTUI 50S RIBOSOMAL SUBUNITS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。H.将研究含有氯霉素和茴香霉素的抗生素抗性突变的marismortui 50 S核糖体亚基。RNA点突变不会立即发生在两种药物的已知结合位点，而是发生在远离抗生素结合位点的一个或多个碱基处。因此，这些实验将检验这样的假设，即细微的结构重排可能保持肽键形成所必需的特征，但改变了药物的结合位点。新的抗生素吉罗林，尼格霉素，和13-脱氧tedanalone，其中有以前未确定的结合位点和未知的机制，抑制蛋白质合成终止也将进行研究

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structures of H. marismortui 50S ribosomal subunits containing antibitoic resistance mutations for chloramphenicol and anisomycin will be studied. The RNA point mutations do not occur immediately at the known binding sites for either drug, but occur one or more bases distant to the antibiotic binding site. Thus these experiments will test the hypothesis that subtle structural rearrangements may maintain features necessary for peptide bond formation but change the binding site for the drug. Novel antibiotics girolline, negamycin, and 13-deoxytedanolide, which have previously unidentified binding sites and unknown mechanisms of inhibiting protein synthesis termination will also be studied