STRUCTURE OF A RAD4-RAD23 COMPLEX BOUND TO DNA

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。RAD 4（人着色性干皮病C的酵母同系物）-RAD 23复合物在全局基因组NER途径的早期起作用，与DDB 1-DDB 2平行，以识别DNA损伤，例如6-4光产物和大体积碱基加合物。如何识别各种化学上不同的损伤并将其交给NER修复机制的其余部分是我们试图通过解决与其同源DNA结合的蛋白质复合物的晶体结构来解决的主要问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The RAD4 (yeast homolog of human Xeroderma Pigmentosum C)-RAD23 complex acts early on in the global genome NER pathway, in parallel to DDB1-DDB2, to recognize DNA lesions such as 6-4 photoproduct and bulky base adducts. How a variety of chemically diverse lesions is recognized and handed to the rest of NER repair machinery are the major questions we are attempting to address by solving crystal structure of the protein complex bound to its cognate DNA.