Proposal for Central California 900 MHZ NMR Spectrometer

描述(由申请人提供)：将为加州中部地区建立一个共享的900 MHz核磁共振设施，将设在加州大学伯克利分校的新QB3研究所(定量生物医学研究所)。该仪器将向整个地区的调查人员提供。具体的建议来自加州大学伯克利分校、斯坦福大学、加州大学旧金山分校、加州大学戴维斯分校、劳伦斯·利弗莫尔国家实验室和基因技术公司的调查人员。仪器上的时间将被分配，以最大限度地应用于挑战当前核磁共振能力限制的问题。正在研究的系统将包括大型病毒和核糖体RNA、VS核酶、病毒蛋白质-RNA相互作用、金属蛋白Heine加氧酶、肿瘤坏死因子配体和受体相互作用、细菌转录激活剂、细胞内研究的蛋白质、细胞因子及其受体，以及参与DNA修复的蛋白质及其复合体。该设施的工作人员将为开展最先进的光谱学、脉冲序列及其文档资料库和一般用户指南提供支持。

DESCRIPTION (provided by applicant): A shared 900 MHz NMR facility will be established for the Central California area, to be housed in the new QB3 Institute (Institute for Quantitative Biomedical Research) at the University of California Berkeley. The instrument will be available to investigators throughout the region. Specific proposals are included from investigators at UC Berkeley, Stanford, UC San Francisco, UC Davis, Lawrence Livermore National Lab and Genentech. Time on the instrument will be allocated to maximize its application to problems which challenge the limits of current NMR capability. Systems under study will include large viral and ribosomal RNAs, VS ribozyme, viral protein-RNA interactions, metalloprotein heine oxygenases, TNF ligand and receptor interactions, bacterial transcriptional activators, proteins studied inside cells, cytokines and their receptors, and proteins involved in DNA repair and their complexes. Support for carrying out state of the art spectroscopy, libraries of pulse sequences and their documentation, and general user guides will be provided by staff members in the facility.