A Zeiss Duo Confocal Microscope for Shared Imaging Facility

我们计划收购最近开发的双配置共聚焦显微镜(LSM 5 Duo，蔡司) 支持美国国立卫生研究院资助的马里兰大学学院9名研究人员的研究 医学。这些研究人员都迫切需要高速、多色荧光共聚焦 在光激活、光漂白或远距离Z向运动期间成像。没有所需的设备 能力存在于校园中。蔡司二人组结合了久负盛名的高分辨率光学 LSM 510的切片功能与最新LSM的高速成像功能 5现场直播。两个独立的波束控制系统为 在快速图像采集期间同时进行感兴趣区域的光操纵。各自的主要用户 确认二人组的能力直接和独特地很好地满足了他们的要求。新的 仪器将并入该大学的长期共焦核心设施，它将在那里 更换1994年购买的LSM410，该LSM410既不适合活细胞，也不可靠 现在在该设施的共焦显微镜上占据了大部分时间的实验。之后 评估主要供应商的可能系统配置，设施监督委员会-S也 双方一致认为，这两种配置是满足日益增长的高速需求的唯一配置， 活细胞成像，同时提供对高分辨率点扫描共焦的一致访问。 因此，S院长级别的制度支持非常强大，并在许多人中广泛传播。 拥有NIH支持的广泛研究的部门。二人组在共焦核中的存在 设施将直接和有力地有利于主要用户的研究；更广泛地说，它将增加一个 推进NIH赞助的许多其他研究人员关于 研究活细胞和系统中的生物过程的校园。

We propose to acquire a recently developed, dually configured confocal microscope (the LSM 5 Duo, Zeiss) to support the research of a group of 9 NIH-funded investigators at the University of Maryland School of Medicine. These investigators each have immediate need for high-speed, multi-color fluorescence confocal imaging during photoactivation, photobleaching, or long-distance Z motion. No equipment with the required capabilities exists on campus. The Zeiss Duo combines the well-established, high-resolution optical sectioning capabilities of the LSM 510 with the high-speed imaging capabilities of the more recent LSM 5Live. The two, independent beam-steering systems of the Duo create the synergistic capacity for simultaneous region-of-interest photomanipulation during rapid image acquisition. The Major Users each confirmed that the capabilities of the Duo directly and uniquely well meet their requirements. The new instrument will be incorporated into the long-standing Confocal Core Facility at the University, where it will replace an LSM410 that was purchased in 1994 and is neither suitable nor reliable for the live-cell experiments that now occupy the large majority of time on the confocal microscopes in the Facility. After evaluating possible system configurations from the major vendors, the Facility?s oversight committee also agreed that the Duo presented the only configuration that would meet the increasing demand for highspeed, live-cell imaging while providing consistent access to a high-resolution point-scanning confocal. Accordingly, institutional support is very strong at the Dean?s level and also spread widely among many Departments with extensive NIH-supported research. The presence of the Duo in the Confocal Core Facility would directly and strongly benefit the research of the Major Users; more broadly, it would add an important new capability for advancing the NIH-sponsored research of the many other investigators on campus studying biological processes in living cells and systems.