Brown University Shared DNA Sequencer

描述（由申请人提供）：本申请建议用最先进的机器替换过时的DNA测序技术（制造商于2006年6月30日停产）。该提案的广泛目标是通过将分子遗传学与目前正在收集的密集表型和环境数据相结合，丰富布朗大学和合作机构正在进行的精神病学和行为学研究。该提案的具体目的是用最先进的Applied Biosystems 3130xl遗传分析仪取代不再支持的Applied Biosystems 377 DNA测序仪。获得一种新的遗传分析仪将允许在鉴定药物作用的遗传调节剂方面取得不间断的进展（例如，McGeary等2004），定义了基因对环境相互作用的遗传贡献（例如，Gibb et al，2006）和定义精神病亚型（例如，McGeary等人，2006年）。目前的研究项目将从此次收购中受益，包括许多酒精和尼古丁依赖药物的试验，目的是通过识别预测积极反应（良好治疗结果）和消极反应（副作用）的遗传变异来改善药物治疗。目前正在审查的19项研究提案将受益于获得这一设备。这些项目涵盖多种方法，包括神经成像评估研究，多种滥用药物的人类实验室挑战研究，肥胖，抑郁和焦虑症的评估以及对人类睡眠的遗传影响的调查。

DESCRIPTION (provided by applicant): This application proposes to replace outdated DNA sequencing technology (discontinued by manufacturer on 6/30/06) with a state of the art machine. The broad objectives of this proposal are to enrich ongoing psychiatric and behavioral research at Brown University and collaborating institutions through the integration of molecular genetics with the intensive phenotypic and environmental data currently being collected. The specific aim of the is proposal is to replace the no-longer supported Applied Biosystems 377 DNA Sequencer with a state-of-the-art Applied Biosystems 3130xl Genetic Analyzer. Obtaining a new genetic analyzer would allow uninterrupted progress in identifying genetic moderators of drug's effects (e.g., McGeary et al 2004), defining genetic contributions to gene by environment interactions (e.g., Gibb et al, 2006) and defining psychiatric subtypes (e.g., McGeary et al., 2006). Current research projects that would benefit from this acquisition include numerous trials of medications for alcohol and nicotine dependence with the goal of improving pharmacological treatments through the identification of genetic variation that predicts positive response (good treatment outcome) and negative response (side effects). There are 19 research proposals currently under review that would benefit from access to this equipment. These projects span multiple methodologies and include neuroimaging assessment studies, human lab challenge studies of multiple drugs of abuse, assessments of obesity, depression and anxiety disorders and investigations of genetic influences on human sleep.