Network for Premature Birth Research Analytical Core

这项提议是为了创建一个分析核心，它将服务于基因组和蛋白质组学 早产研究网络。分析核心将具有五个组件，这些组件将满足 需要1)样品处理、储存和分发/检索；2)高通量基因分型和 基因研究的解释；3)微阵列分析和数据挖掘；4)蛋白质组学、脂类组学和 氧化应激评估；以及5)生物信息学和统计学。此核心的每个组件都将 由一名在具体方法和数据解释方面具有专业知识的资深调查员监督。 分析核心1职能的全面协调将由首席调查员负责 世卫组织还将担任样品处理、储存、分发/回收部门的主任， 行政单位(Jerome F.Strauss，III，M.D.，Ph.D.)，与构成部分的设施协调工作 董事(基因分型和基因分析：理查德·S·斯皮尔曼博士；微阵列：唐·鲍德温博士； 蛋白质组学/脂质组学与氧化应激：伊恩·布莱尔，博士：生物信息学与统计学 Fenstermacher博士)世卫组织将担任分析核心指导委员会。《校长》 调查员将担任指导委员会主席，并与其他调查员和 参与这项计划的NI.C.H.D.工作人员。拟议的核心是建立在 宾夕法尼亚大学研究早产遗传学，复杂性状遗传学， 蛋白质组学、氧化应激生物标志物研究和微阵列分析。现有的设施是 准备好适应高通量的分析。此外，核心关键人员可以提供专家 研究设计和数据分析方面的建议。围绕混合映射的使用而构建的概念协议 以及传递不平衡检验，以确定血统对早产和新生儿的影响 早产后的结果以及关联和关联研究，以确定特定的风险促进因素 和保护性基因，以及原产地的亲本效应。

This proposal is submitted to create an Analytical Core, which will serve the Genomic and Proteomics Network for Premature Birth Research. The Analytical Core will have five components that will meet the needs for 1) sample processing, storage and distribution/retrieval; 2) high-throughput genotyping and interpretation of genetic studies; 3) microarray analysis and data mining; 4) proteomics, lipidomics and assessment of oxidative stress; and 5) Bioinformatics and statistics. Each component of this Core will be supervised by an established investigator with expertise in the specific methodology and data interpretation. The overall coordination of Analytical Core1 functions will be the responsibility of the Principal Investigator who will also serve as the Director of the sample processing, storage, distribution/retrieval component and administrative unit (Jerome F. Strauss, III, M.D., Ph.D.), working in concert with the component facility directors (Genotyping and genetic analysis: Richard S. Spielman, Ph.D.; Microarray: Don Baldwin Ph.D.; Proteomics/lipidomics and oxidative stress: Ian Blair, Ph.D.: Bioinformatics and statistics: David Fenstermacher, Ph.D.) who will serve as the Analytical Core Steering Committee. The Principal Investigator will serve as the Steering Committee Chair and the interface with the other investigators and N.I.C.H.D. staff participating in this program. The proposed Core builds on established strengths of the University of Pennsylvania in the study of the genetics of preterm birth, the genetics of complex traits, proteomics, the study of oxidative stress biomarkers, and microarray analysis. The existing facilities are prepared to accommodate high-throughput assays. In addition, the Core key personnel can provide expert advice in study design and data analysis. A Concept Protocol built around the use of admixture mapping and the transmission disequilibrium test to identify the impact of ancestry on preterm birth and neonatal outcomes after preterm delivery as well as association and linkage studies to identify specific risk promoting and protective genes, and parent of origin effects.