Core--Cell culture and physiology

细胞培养和生理学核心将是一个集中的设施，将提供原代肺泡上皮2型细胞和次级肺泡上皮细胞系的五个项目中的每一个在程序项目，以及进行生理学实验，利用 离体肺灌流模型。细胞培养设施的集中化将确保 高质量和纯度的肺泡上皮细胞的持续供应可用于五个人中的每一个。 项目该核心将描述本项目中使用的初级和次级肺泡上皮细胞的特征。细胞培养机构人员在肺泡上皮2型细胞的分离以及一般细胞培养技术方面具有丰富的经验。细胞培养设施的巩固将提供一种分离和培养肺泡上皮细胞的经济手段。核心还将进行生理学实验，以测量肺泡液体重吸收率和主动Na+转运利用隔离灌注肺模型。生理学实验的集中化将降低在各个项目中进行这些实验的成本，更重要的是保持这些实验进行的精度和准确性。

The Cell Culture and Physiology Core will be a centralized facility that will provide primary alveolar epithelial type 2 cells and secondary alveolar epithelial cell lines to each of the five projects within the program project, as well as conduct physiology experiments utilizing the isolated perfused lung model. Centralization of the cell culture facilities will ensure that a continuous supply of high quality and purity alveolar epithelial cells is available to each of the five projects. The core will characterize the primary and secondary alveolar epithelial cells used in this program project. The cell culture facility personnel have extensive experience in the isolation of alveolar epithelial type 2 cells, as well as general cell culture techniques. The consolidation of the cell culture facilities will provide an economical means of isolating and culturing alveolar epithelial cells. The core will also conduct physiology experiments to measure the rate of alveolar fluid reabsorption and active Na+ transport utilizing the isolated-perfused lung model. Centralization of the physiology experiments will reduce the cost of conducting these experiments within the individual projects and more importantly maintain the precision and accuracy in which these experiments are conducted.