Core-- Electrophysiology Core

电生理学核心将为体外电生理学研究提供集中服务，包括体外脑片的准备、膜片钳记录、细胞外记录以及相关的图像和数据处理及分析。四个项目中的五个AIMS将利用这一核心设施。全细胞膜片钳记录将来自大鼠脑片的基底前脑神经元(方案2和3)，腹外侧视前(VLPO)神经元(方案3)和小鼠结节，蓝斑和基底前脑神经元(方案5)。此外，海马场电位的细胞外记录将从CA1区获得(项目2和4)。这一资源的集中化使体外电生理记录技术可供多名研究人员在促进所有项目记录条件标准化的环境中使用。

The Electrophysiology Core will provide centralized services for in vitro electrophysiological studies, including preparation of the in vitro brain slices, patch-clamp recordings, extracellular recordings, and the associated image and data processing plus analysis. Five aims in four projects will utilize this Core facility. Whole-cell patch-clamp recordings will be obtained from in vitro rat brain slices from basal forebrain neurons (Projects 2 & 3), ventrolateral preoptic (VLPO) neurons (Project 3) and from mouse tuberornarnmUlary, locus coeruleus and basal forebrain neurons (Project 5). In addition, extracellular recordings of hippocampal field potentials will be obtained from the CA1 region (Projects 2 & 4). The centralization of this resource makes the technique of in vitro electrophysiological recording available to multiple investigators in an environment that facilitates standardization of recording conditions across all projects.