REGULATION OF OVARIAN SURFACE EPITHELIAL CELLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 卵巢表面上皮（OSE）是卵巢周围的单层上皮，占卵巢质量的不到1/1，000，但引起近90%的女性卵巢癌。 卵巢癌的终生风险几乎为1/50，OSE细胞必须被认为是最容易转化的细胞之一。 本研究以非人类灵长类动物为模型，研究恒河猴OSE（RhOSE）细胞生物学特性。 初步研究建立了RhOSE细胞培养物，并与人OSE细胞培养物进行了比较分析。 这些数据表明，人和恒河猴细胞之间具有高度相似性，共同的基因表达与来自实验室或家畜（如小鼠、大鼠、绵羊或猪）的OSE细胞不同。 该项目的长期目标是使用细胞培养、基因微阵列和其他分子技术来确定RhOSE增殖和存活是如何在体外调节的。 其他研究已经开始检查体内OSE的基因表达，通过收集猕猴卵巢月经周期的定义阶段。 这些研究将检查排卵前后OSE的正常命运，以及在卵巢功能的背景下OSE增殖和死亡的调节。 这些努力的结果可能表明哪些生理事件促进OSE转化，并可能转化为临床应用，以开发更有效的方法来预防，检测和治疗卵巢癌。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ovarian surface epithelium (OSE) is a monolayer surrounding the ovary that comprises less than 1/1,000th of the ovarian mass, yet gives rise to nearly 90% of ovarian cancers in women. With a life-time risk of ovarian cancer almost 1 in 50, OSE cells must be considered among the most highly prone to transformation. This project was developed to consider a nonhuman primate model to investigate rhesus macaque OSE (RhOSE) cell biology in vitro and in vivo. Initial studies established RhOSE cell cultures and performed comparative analysis with human OSE cell cultures. These data showed high similarity between human and rhesus cells, with common gene expression not shared with OSE cells derived from laboratory or domestic animals, such as mouse, rat, sheep, or pig. The long-term goal of this project is to use cell culture, gene microarray, and other molecular techniques to determine how RhOSE proliferation and survival are regulated in vitro. Additional studies have begun to examine gene expression in the OSE in vivo, by collecting macaque ovaries from defined phases of the menstrual cycle. These studies will examine the normal fate of the OSE before and after ovulation and the regulation of OSE proliferation and death in the context of ovarian function. The results of these efforts may indicate which physiological events promote OSE transformation and could translate into clinical applications to develop more effective methods for prevention, detection, and treatment of ovarian cancer.