BIOMARKERS OF BRAIN PATHOLOGY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个项目的目的是开发使用质谱学来观察可卡因滥用引起的大脑化学变化的轮妥醇，使用猴子模型和使用小鼠模型实验诱导更年期。在本年度，重点一直放在开发测试方案上，以测量自行服用可卡因的猴子大脑中神经递质及其代谢物的变化。样品的采集采用微透析技术。这些数据将与从磁共振波谱(MRS)获得的数据进行比较，MRS是一种MRI神经成像形式，可以在体内产生特定大脑区域的神经化学变化光谱。这种方法将验证MRS在未来对药物摄入引起的神经化学变化的研究中的使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is designed to develop rotocols using mass spectroscopy to look at changes in brain chemistry resulting from cocaine abuse using a monkey model and experimentally induced menopause using a mouse model. During the current year, focus has been on developing assay protocols to measure changes in neurotransmitters and their metabolites in the brains of monkeys self-administering cocaine. Samples are collected using microdialysis. These data will be compared to those obtained from the same monkeys obtained from magnetic resonance spectroscopy (MRS) a form of MRI neuroimaging that can generate spectra of neurochemical changes in specific brain regions in vivo. This approach will validate the use of MRS in future studies of neurochemical changes resulting from drug intake.