PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目前对具有特定基因型的动物种群存在显著需求，这不能通过从野生动物进口或通过选择性育种鉴定和繁殖有价值的创始动物来满足。 携带主要组织相容性复合体（MHC）1类等位基因A*01的印度恒河猴特别需要用于疫苗开发研究。 本研究的目的是证明，辅助生殖技术（ART）可以应用于快速，有效的繁殖的一个有价值的创始人动物使用现有的技术，从而建立一个有效的方法来满足动物的生物医学研究界的要求。 关注纯合创始雄性的基本原理是该动物产生的所有后代均为Mamu-A*01阳性。 此外，如果使用A*01等位基因的杂合携带者作为卵母细胞供体，则50%的后代将是等位基因纯合的，从而产生额外的创始动物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There is currently a significant need for populations of animals with specified genotypes, which cannot be satisfied by the importation of animals from the wild, or by the identification and propagation of valuable founder animals by selective breeding. Indian-origin, rhesus macaques carrying the major histocompatability complex (MHC) class 1 allele, A*01, are particularly needed for vaccine development research. The objective of this research is to demonstrate that the assisted reproductive technologies (ARTs) can be applied to the rapid, efficient propagation of a valuable founder animal using existing technology, thereby establishing an effective approach to satisfying animal requirements of the biomedical research community. The rationale for focusing on a homozygous, founder male is that all offspring produced by this animal will be Mamu-A*01 positive. Moreover, if the heterozygous carriers of the A*01 allele are used as oocyte donors, 50% of the offspring will be homozygous for the allele, creating additional founder animals.