BIOLOGY OF THE PRIMATE OVARIAN SURFACE EPITHELIUM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 上皮性卵巢癌的病因知之甚少，部分原因是它对灵长类动物(和鸡)的独特性限制了动物模型的可获得性。此外，正常灵长类卵巢表面上皮(OSE)的生物学还没有得到有效的研究，因此关于其转化原因的线索并不是基于经验观察。由于很难获得合适的人体组织(来自生殖年龄的女性的健康卵巢)以及人类研究的实用和伦理限制，我们选择非人类灵长类猕猴作为人类系统的替代模型，其基础是：(1)共享生殖和卵巢生理；(2)共享非灵长类哺乳动物常见的基因表达；(3)关于人和非人类灵长类动物卵巢上皮癌的报道，但不是其他哺乳动物，强调了灵长类OSE的潜在独特性。我们的目标是用免疫组织化学方法研究灵长类OSE在月经周期不同阶段的特征；确定卵巢因素(雌激素和孕酮)是否在体内动态调节OSE；以及确定OSE是否是卵巢功能和健康的重要组成部分。该项目将建立一个了解灵长类OSE的基本基线，并为基于假设的实验评估卵巢癌的风险和新的治疗策略提供一个模型系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The etiology of epithelial ovarian cancer is poorly understood, in part because its uniqueness to primates (and chickens) limits the availability of an animal model. In addition, the biology of the normal primate ovarian surface epithelium (OSE) has not been effectively studied, so clues to the causes of its transformation are not based on empirical observation. Due to the difficulty in obtaining appropriate human tissue (healthy ovaries from reproductively aged women) and the practical and ethical limitations on human studies, we selected the nonhuman primate rhesus monkey as a surrogate model for the human system on the basis of: (1) shared reproductive and ovarian physiology; (2) shared gene expression that is not common to nonprimate mammals; and (3) reports of epithelial ovarian cancer in human and nonhuman primates, but not other mammals, that emphasize the potential uniqueness of the primate OSE. Our aims are to characterize the primate OSE at distinct phases of the menstrual cycle using immunohistochemical methods; to determine whether ovarian factors (estrogen and progesterone) dynamically regulate the OSE in vivo; and to establish whether the OSE is an essential component of ovarian function and health. This project will establish a fundamental baseline to understand the primate OSE and provide a model system for hypothesis-based experiments to evaluate risks for ovarian cancer and novel therapeutic strategies.