DARTMOUTH COL COBRE: P1: REGULATION OF BIOFILM DEVELOPMENT ON EPITHELIAL CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 生物膜是临床、工业和自然环境中发现的附着在表面的微生物群落。大量研究表明，生物膜细菌与浮游细菌在形态和生理上是不同的，在基因和蛋白质表达谱上也不同。我们提出，生物膜的形成是一个受良好调控的发育系统。如果生物膜的形成类似于其他发育系统，我们假设应该有不同的调控途径来控制浮游生物膜生长之间的过渡，并且这个调节级联反应应该受到环境信号的控制。在最初的提案中，我们描述了对SADARS三要素监管系统的研究。我们之前已经报道过这些研究。我们已经将这项工作扩展到包括其他几个在早期生物膜发育中起关键作用的基因，包括SADC和BIFA。我们在这些新发现的基因方面的进展概述如下。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biofilms are surface-attached microbial communities found in clinical, industrial and natural environments. Numerous studies suggest that biofilm bacteria can be distinguished from their planktonic counterparts in terms of morphology and physiology, and also differ in gene and protein expression profiles. We have proposed that the formation of biofilms is a well-regulated developmental system. If biofilm formation is analogous to other developmental systems we hypothesize that there should be distinct regulatory pathways that control the transition between planktonic and biofilm growth, and this regulatory cascade should be controlled in response to environmental signals. In the original proposal we described studies of the SadARS three-component regulatory system. We have reported those studies previously. We have extended this work to include several other genes with key roles in early biofilm development, including SadC and BifA. Our progress on these newly discovered genes is outlined below.