RAPID MIXING SYSTEM FLOW CYTOMETER

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们已经开发了一种具有亚秒级分辨率的停流流式细胞仪，用于细胞活化的动力学分析。 该仪器将流式细胞术的应用扩展到与细胞表面受体相互作用或可以连接到珠子的重要生物分子。该装置是可操作的，并可用于反应到100毫秒的时间分辨率。它目前被用于粘附和信号转导配体的分析和酶测定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed a stopped-flow flow cytometer with subsecond resolution to be used in kinetic analysis of cell activation. This instrument is extending the application of flow cytometry to important biological molecules that interact with cell surface receptors or can be linked to beads. The device is operational, and can be used for reactions to100 msec time resolution. It is presently being used for analysis of adhesion and signal transduction ligands and enzymatic assays.