XENOPUS SPINDLE MATRIX PROTEOME

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 鸟苷三磷酸酶RAN(RanGTP)可以刺激V型中间丝蛋白Lamin B组装成膜质有丝分裂纺锤体基质，这是正确的有丝分裂纺锤体组装所必需的。我们在这里报道，分离的纺锤体基质含有已知的纺锤体组装因子以及对生物发育至关重要的信号分子和转录因子。有丝分裂的纺锤体基质既是基于MT的纺锤体组装的拉伸结构，也是有丝分裂中连接纺锤体组装因子和细胞命运决定因素的框架。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Guanosine triphosphatase Ran (RanGTP) can stimulate assembly of the type V intermediate filament protein, lamin B, into a membranous mitotic spindle matrix, which is required for proper mitotic spindle assembly. We report here that the isolated spindle matrix contains known spindle assembly factors as well as signaling molecules and transcription factors essential for organismal development. The mitotic spindle matrix is both a tensile structure for MT-based spindle assembly and a framework for tethering spindle assembly factors and cell fate determinants in mitosis.