PROTEIN INTERACTIONS WITH YEAST HHO1P

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 酵母有一种蛋白质与较高细胞组蛋白H1在结构和功能上具有显著的相似性。我们假设，对于核心组蛋白，“尾部”和(仅对于酵母H1)内部的“连接物”多肽代表蛋白质-蛋白质相互作用模块，其活性将被证明受特定的翻译后修饰控制。我们寻求确定这样的互动伙伴。全基因组亲和标记项目已经确定了几个候选者，但目前很难评估他们的有效性。因此，我们提出了几种互补的方法，包括全基因组的双杂交实验。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Yeast have one protein having significant structural and functional similarity to higher cell histone H1. We hypothesize that, as for the core histones, the "tail" and (for yeast H1 only) internal "linker" peptides represent protein-protein interaction modules whose activity will prove to be controlled by specific posttranslational modifications. We seek to identify such interacting partners. The genome-wide affinity-tagging project has identified several candidates, but their validity is difficult to assess at present. Thus, we propose several complementary approaches, including a genome-wide 2-hybrid experiment.