ELECTRODE POSITIONING BY ACCESS ADMITTANCE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 自参考技术检测到的化学信号不可避免地与距离有关，这是分析物在细胞外基质中扩散的结果。显然，这需要在距电池特定距离处可重复地放置电极。不幸的是，手动放置探头可能相当困难，并且仅限于细胞的x-y平面中的高对比度边界。提供一种用于确定距离的仪器方法不仅将允许从z轴分析单元，而且还将提供用于量化测量距离的可重复的手段，从而允许对单个单元上的多个点或多个单元之间的多点进行比较。我们的目标是实现阻抗反馈来测量和控制样品-探头距离。在短期内，这项技术将应用于流量测量的距离控制。从长远来看，这项技术将允许我们将扫描显微镜添加到我们的分析工具箱中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Chemical signals detected by the self-referencing technique are inevitably distance dependent, resulting from diffusion of the analyte through the extracellular matrix. Obviously this necessitates the reproducible placement of electrodes at specific distances from the cell. Unfortunately, manual placement of the probe can be quite difficult and is limited to the high contrast boundary in the x-y plane of the cell. Providing an instrumental method for determining distance would not only allow analysis of the cell from the z axis, it would additionally provide a reproducible means for quantifying the distance at which measurements are taken, allowing comparison of multiple points on a single cell or among multiple cells. Our goal is the implementation of impedance feedback to measure and control the sample-probe distance. In the short-term this technology will be applied to the control of distance for flux measurements. In the long-term this technology will allow us to add scanning microscopy to our analytical toolbox.