LUMINESCENT IMAGING: TARGETING ATP

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 发光（光子发射）报告提供了不同的角度对细胞活动比更传统的荧光报告  后者需要激励频率来反应。 发光报告物，虽然远没有那么多样化，但依赖于两个分子之间的化学相互作用，其中缔合依赖于待测量的靶标。 在这里开发的情况下，我们希望通过发射光子的ATP/荧光素酶反应来记录ATP的细胞活性。荧光素是膜可渗透的，并且由于荧光素酶是一种蛋白质，如果合适的载体可用，它可以靶向和表达。 这一子项目的目标是获取这些载体，并在我们的光子计数/成像系统中对其进行测试（见SPID 0094）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Luminescent (photon emitting) reporters offer different perspectives on cell activity than the more conventional fluorescent reporters  the latter requiring an excitation frequency to react. Luminescent reporters, although far less diverse, depend upon and chemical interaction between two molecules where the association is dependent on the target to be measured. In the case being developed here we wish to record the cellular activity of ATP through the photon emitting luciferin/luciferase reaction. Luciferin is membrane permeable and as luciferase is a protein it can targeted and expressed if a suitable vector is available. Acquisition of such vectors, and their testing in our photon counting/imaging systems (see SPID 0094) are the goals of this subproject.