FLOW CYTROMETRY

流式细胞术 流式细胞术共享资源一直是UCSD癌症中心的一个组成部分， 1990.它的存在是为了提供同行评审，资助研究人员从所有部门的癌症中心，准备 对来自临床样品、动物和动物的解离细胞群进行高速分析和分选 实验和细胞培养研究。该资源提供的服务如下： 分析流式细胞术：使用FACSCalibur？流式细胞仪和新购买的FACSAria？ 流式细胞仪FACSCalibur是一款台式流式细胞仪，可用于分析多达四种颜色 of fluorescence荧光. FACSAria <$是一种三激光流式细胞仪，可进行多达11个 荧光的颜色，沿着与前向和侧向角光散射。FACSAria利用FACSDiva 软件，它具有许多高级功能，如双指数缩放，自适应门控和自动化 荧光光谱补偿FACSCalibur <$流式细胞仪计算机系统使用 用于列表模式数据记录和分析的CELLQuest <$软件。这些服务使调查人员能够 分析细胞群的细胞周期状态、表面和细胞质抗原表型、细胞凋亡，或 转基因的表达（例如在用绿色荧光蛋白[GFP]转导后）。

Flow Cytometry The Flow Cytometry Shared Resource has been an integral component of the UCSD Cancer Center since 1990. It exists to provide peer-review, funded investigators from all Divisions of the Cancer Center with ready access to high-speed analysis and sorting of dissociated cell populations from clinical samples, animal experiments and cell culture studies. The services available from this Resource are as follows: Analytical Flow Cytometry: Utilizes a FACSCalibur¿ flow cytometer and a newly purchased FACSAria¿ flow cytometer. The FACSCalibur¿ is a bench-top flow cytometer that can be used for analysis of up to fourcolors of fluorescence. The FACSAria¿ is a three-laser flow cytometer for analyses capable of up to eleven colors of fluorescence, along with forward and side-anglejight scatter. The FACSAria¿ utilizes FACSDiva¿ software, which has many advanced features, such as biexponential scaling, adaptive gating, and automated fluorescence spectral compensation. The FACSCalibur¿ flow cytometer computer system uses CELLQuest¿ software for list-mode data recording and analysis. These services allow investigators to analyze cell populations for cell cycle status, surface and cytoplasmic antigen phenotype, apoptosis, or expression of a transgene (e.g. following transduction with green fluorescent protein [GFP]).