CYSTEINE TARGETED REDOX REGULATION OF PROTEINS HOW WIDESPREAD IS REGULATION BY SULPHINIC ACID FORMATION?

缺血性心脏病是西方世界最常见的死亡原因，在英国每年有超过15万人死亡。当流向心脏某个区域的血液因冠状动脉狭窄或阻塞而减少时（缺血），心肌就会受伤，如果阻塞严重，心肌就会死亡（梗死）。这基本上就是心脏病发作时发生的事情。因此，如果心肌要存活，那么阻塞的冠状动脉中的血流必须迅速恢复（再灌注）。在缺血和再灌注过程中，产生有毒形式的氧（自由基），破坏细胞的关键成分，包括蛋白质。我们已经开发了几种新的方法，使我们能够识别在缺血和再灌注期间被自由基氧化的心脏蛋白质。这些蛋白质的鉴定将有助于我们了解缺血和再灌注损伤的机制，并确定可能的治疗策略，以对抗心脏病发作期间的损伤。

Ischaemic heart disease is the most common cause of mortality in the Western world and accounts for more than 150,000 deaths per year in the U.K. When blood flow to a region of the heart is reduced by narrowing or blocking of a coronary artery (ischaemia), the heart muscle becomes injured and, if the blockage is severe, the heart muscle will die (infarct). This is essentially what happens during a heart attack. It is essential therefore that the flow in the blocked coronary artery is rapidly restored (reperfusion) if the heart muscle is to survive. During ischaemia and reperfusion toxic forms of oxygen (free radicals) are produced that damage crucial components of the cell, including proteins. We have developed several new methods that allow us to identify heart proteins that are oxidised by free radicals during ischaemia and reperfusion. The identification of these proteins will help us understand the mechanisms of injury during ischaemia and reperfusion and identify possible therapeutic strategies to combat injury during a heart attack.