DNA MICROARRAY ANALYSIS OF GENE EXPRESSION IN RESPONSE TO TRAUMATIC INJURY IN TH

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本项目的目的是优化多聚甲醛固定的猴脑组织中基因表达的分析方法。该项目的具体目的是(1)修改现有的从固定脑组织中提取总RNA的方法，(2)优化DNA微阵列和实时RT-PCR的方法，以使用从多聚甲醛固定的脑组织中提取的RNA，(3)分析创伤后6个月和1年获得的固定组织中恒河猴脑的差异基因表达。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project was to optimize protocols for the analysis of gene expression in paraformaldehyde fixed monkey brain tissue. The specific aims of the project were (1) To modify available protocols to extract total RNA from fixed brain tissue, (2) To optimize protocols for DNA microarray and real time RT-PCR to use RNA extracted from paraformaldehyde fixed brain tissues, and (3) To analyze differential gene expression in the rhesus monkey brain in fixed tissues obtained at six months and one year after traumatic brain injury.