Optical Microscopy Core

光学形态核心将在支持中心的总体目标方面发挥重要作用。 这个核心的具体目标有三个方面。第一，提供可靠、可访问和最先进的 向所有中心成员提供微观技术，促进他们对G1细胞信号的研究 瀑布。第二，教育和培训中心成员使用基本的和复杂的 细胞成像方法。重点将放在提供技术指导以及 教育教职员工这种方法如何扩大他们的科学研究的范围和广度 程序。第三，最先进的光学成像技术在全球范围内的发展和应用 组织/细胞，包括：活细胞的高分辨率实时计算机/视频成像；共聚焦 显微结合计算机三维图像重建；荧光共振能 用于测量动态蛋白质相互作用的转移(FRET)应用；荧光恢复 光漂白后(FRAP)，可以定量测定蛋白质的募集/周转；表达 以及促进特定信号的研究和定位的基于荧光的生物探针的使用 包括蛋白质和脂类在内的分子，以及特定的光激活物质的开发和应用 笼状化合物，允许精确的时间和空间激活所需的信号 活细胞中的分子。

The Optical Morphology Core will play an essential role in supporting the overall goals of the Center. The Specific Aims of this core are three-fold. First, to provide reliable, accessible, and state-of-the-art microscopic technology to all Center members that will facilitate their study of Gl cellular signaling cascades. Second, to educate and train Center members in the use of both basic and sophisticated cellular imaging methods. Emphasis will be placed on providing technical instruction as well as educating faculty about how such approaches can expand the scope and breadth of their scientific programs. Third, development and application of state-of-the-art optical imaging technologies to Gl tissues/cells including; high resolution, real-time computer/video imaging of live cells; confocal microscopy coupled with computer-based 3-D image reconstruction; Fluorescence Resonance Energy Transfer (FRET) applications to measure dynamic protein-protein interactions; Fluorescence Recovery After Photobleaching (FRAP) that allows the quantitation of protein recruitment/turnover; expression and use of fluorescence-based bioprobes that facilitates the study and localization of specific signaling molecules including both proteins and lipids; and the development and application of specific photoactivatable caged-compounds that allow a precise temporal and spatial activation of desired signaling molecules in live cells.