CELL MIGRATION CONSORTIUM PROJECT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 使用FCS来确定下列分子在迁移细胞中的浓度、聚集状态和相互作用(按优先顺序)。(帕西林、α-放线菌素；FAK和帕西林；GIT1和帕西林；GIT1和Pix、Rac或Pak)。用FCS测定浓度和聚集度。(1)粘连及其周围的分子数量；(2)异位表达，然后内源性表达水平；(3)测定粘连及其周围的复杂分子的数量；(4)用FCS(Pak，Rac)检测可溶性成分的激活；(5)确定粘连形成和分散时聚集和相互作用的变化。并使用FCS确定单个蛋白质和相互作用蛋白质的动力学

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Use FCS to determine the concentrations, aggregation states, and interactions of the following molecules (in priority order) in migrating cells. (paxillin, a-actinin; FAK and paxillin; GIT1 and paxillin; GIT1 and Pix, Rac, or Pak). Determine concentrations and degree of aggregation by FCS. (1) number of molecules in and around adhesions; (2) ectopic expression and then endogenous expression levels; (3) detrmine the number of complexed molecules in and around adhesions; (4) detect activation of soluble components by FCS (paK, Rac); (5) determine changes in aggregation and interactions as adhesions form and disperse. and determine dynamics of single and interacting proteins using FCS