SALIVARY GLAND EXOSOME PROTEOME

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 唾液是一种口服液，富含一千多种独特的蛋白质，这些蛋白质由几种途径分泌，可能包括外切体。外切小体是在构成分泌的多囊内小体中由内体起源的膜结合小泡。一种基于质谱学的鸟枪式蛋白质组学方法被用来探索唾液中发现的许多蛋白质来自外切体的可能性。利用MudPIT(多维蛋白质识别技术)，我们对人腮腺唾液外体部分中的491个蛋白质进行了编目。腮腺分泌的外泌体可能携带对口腔和全身疾病的诊断、预后和治疗有用的信息蛋白标记物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Saliva is an oral fluid rich in over a thousand unique proteins which are secreted by several pathways, possibly including exosomes. Exosomes are membrane-bound vesicles of endosomal origin within multivesicular endosomes that are constitutively secreted. A mass spectrometry-based shotgun proteomics approach was employed to explore the possibility that many of the proteins found in saliva are derived from exosomes. Using MudPIT (multidimensional protein identification technology), we catalogued 491 proteins in the exosome fraction of human parotid saliva. Parotid glands secrete exosomes that may carry informative protein markers useful in the diagnosis, prognosis and treatment of oral and systemic diseases.