BINDIN FROM A SEA STAR

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 发育进化的遗传基础包括编码精子和卵子表面表达的蛋白质的基因。以前对精子顶体蛋白结合蛋白的研究有助于描述海胆配子亲和性和物种形成的适应性进化。除了棘球虫总科之外，没有证据表明bindin在分类中的表达，这使得这类研究仅限于海胆，并导致了bindin可能是海胆特有的分子的说法。在这里，我们描述了在广播产卵的小行星海星(Patiria Mini Ata)中编码结合蛋白的基因。我们描述了全长Bindin基因及其单内含子的序列和结构域结构。与海胆相比，小型海胆的结合蛋白更大，但这两种分子 共享其结构域结构的几个一般特征和两个结构域的一些序列特征。我们的结果将已知的结合素的进化历史从中生代(在冠群海胆中)延伸到了早古生代(也是海胆的共同祖先)，并为理解结合素分子进化在有性繁殖中的作用提供了新的机会。 海星的选择、生活史进化和物种形成。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The genetic basis for the evolution of development includes genes that encode proteins expressed on the surfaces of sperm and eggs. Previous studies of the sperm acrosomal protein bindin have helped to characterize the adaptive evolution of gamete compatibility and speciation in sea urchins. The absence of evidence for bindin expression in taxa other than the Echinoidea has limited such studies to sea urchins, and led to the suggestion that bindin might be a sea urchin-specific molecule. Here we characterize the gene that encodes bindin in a broadcast-spawning asterinid sea star (Patiria miniata). We describe the sequence and domain structure of a full-length bindin cDNA and its single intron. In comparison to sea urchins, P. miniata bindin is larger but the two molecules share several general features of their domain structure and some sequence features of two domains. Our results extend the known evolutionary history of bindin from the Mesozoic (among the crown group sea urchins) into the early Paleozoic (and the common ancestor of eleutherozoans), and present new opportunities for understanding the role of bindin molecular evolution in sexual selection, life history evolution, and speciation among sea stars.