STRUCTURES OF PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目前正在对需要同步辐射的多种结构进行调查。其中包括多达42个蛋白质亚基的1.1和1.2个丙二醛复合体(蛋白酶体激活剂复合体)，对病毒衣壳组装和动力学至关重要的HIV-1 Gag衍生结构域的结构，在HIV发芽中起作用的细胞蛋白质和复合体，核小体重塑和重组复合体，以及血红素生物合成的酶。在许多情况下，晶体目前已准备好进行数据收集，而在其他情况下，晶体很可能在今后几个月/几年内获得。在某些情况下，我们需要同步辐射，因为衍射本身就很弱，或者因为晶体很小。其他时候，我们需要高分辨率的可靠性，查看可能指导未来药物开发努力的复合体的细节。其他时候，我们需要可调谐的辐射，以便获得相位信息或在差分图中定位反常散射中心。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Multiple structures that require synchrotron radiation are currently under investigation. These include 1.1 and 1.2 MDa complexes of up to 42 protein subunits (proteasome activator complexes), structures of HIV-1 Gag-derived domains that are important for viral capsid assembly and dynamics, cellular proteins and complexes that function in HIV budding, nucleosome remodeling and reorganizing complexes, and enzymes of heme biosynthesis. In many cases crystals are currently ready for data collection while in other cases crystals are likely to be obtained within the next few months/years. In some cases we need synchrotron radiation because the diffraction is inherently weak or because crystals are small. Other times we need high resolution to reliability see details of complexes that might guide future effort at drug development. Other times we need tunable radiation in order to obtain phase information or to localize anomalous scattering centers in difference maps.