ANALYTICAL TECHNIQUES FOR STRUCTURE ANALYSIS OF GLYCOSAMINOGLYCANS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这是一门新课程，2008年8月25日至27日首次授课。与会者学习了糖胺多聚糖(GAG)结构表征的基本技术，包括使用适当的酶将GAG多糖解聚为其二糖组分。二糖用离子交换层析分离，产物用紫外可见分光光度法检测，用2-氰基乙酰胺衍生化，荧光检测。在另一项实验中，用亚硝酸对糖胺多聚糖进行水解，并对产物进行分析。还介绍了从糖胺多聚糖中提取的二糖和低聚糖的质谱学分析技术。基本的生化技术经验是参加的先决条件。共有8名参与者，其中7名来自非营利组织，1名来自行业。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This was a new course, which was taught for the first time August 25-27, 2008. Participants learnt basic techniques for the structural characterization of glycosaminoglycans (GAGs), which included depolymerization of the GAG polysaccharides to its disaccharide constituents using appropriate enzymes. The disaccharides were separated by ion exchange chromatography and the products detected by U.V. The disaccharides were derivatized with 2-cyanoacetamide and were detected with fluorescent detection. In a separate experiment, the glycosaminoglycans were hydrolyzed by nitrous acid and the products analyzed. Mass spectrometry techniques for analysis of di- and oligosaccharides derived from glycosaminoglycans were also covered. Experience with basic biochemical techniques is a prerequisite for participation. A total of 8 participants with 7 from non-profit and 1 from industry.