TRANSEPITHELIAL ION TRANSPORT IN MOSQUITO HINDGUT CONTROLLED BY PEPTIDE HORMONE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们建议研究蚊子排泄物上皮细胞跨上皮离子转运的机制以及一种多肽激素离子转运多肽对其的调控。非洲疟疾蚊子冈比亚按蚊生活在淡水中，需要通过后肠和肛门乳头主动吸收离子。成蚊在采血过程中和采血后的短时间内处理大量的液体食物。我们假设离子转运肽(ITP)是控制蚊子渗透压调节功能的主要参与者，因为它被称为蝗虫。AN中离子转运肽的基础生物学。冈比亚将使用各种分子和生化技术进行研究。我们将特别询问ITP亚型何时何地表达和分泌的问题。ITP异构体在马氏管和后肠上的活性将通过测量水分传输速率和电生理方法来研究。拟议的研究将提供内分泌系统如何控制蚊子跨上皮离子运输的知识。了解基本的媒介生物学和生理学将为开发环境友好的杀虫化合物提供合理的途径，有助于控制媒介传播的疾病，改善人类健康。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to investigate mechanisms of transepithelial ion transport in mosquito excretory epithelial cells and its control by a peptide hormone ion transport peptide. African malaria mosquito Anopheles gambiae inhabits in fresh water, requiring active absorption of ions through hindgut and anal papillae. The adult mosquito processes a large amount of the liquid food in a short period of time during and after the blood feeding. We hypothesize that ion transport peptide (ITP) is a major player in the control of mosquito osmoregulatory function as it is known for the locust. Basic biology of the ion transport peptide in the An. gambiae will be studied using various molecular and biochemical techniques. We will specifically ask questions when and where the ITP isoforms are expressed and secreted. The activities of the ITP isoforms on malpighian tubules and hindgut will be studied by measuring the rate of water transport, and by electrophysiological methods. Proposed research will provide the knowledge how the endocrine system controls transepithelial ion transport in mosquitoes. Understanding basic vector biology and physiology will provide rational approaches to develop environmentally benign insecticidal compounds that help to control vector-born diseases, improving human health.