NONHUMAN PRIMATE MODEL FOR TB VACCINES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 加州国家灵长类动物研究中心的动物生物安全3级(ABSL-3)设施已经建成，并为结核分枝杆菌(M.TB)的研究做准备。在恒河猴身上。ABSL-3设施包括两个容纳动物的房间和一个设备齐全的尸检套件，用于全面的病理分析。为研究结核分枝杆菌对兽医和研究支持人员进行了培训。在这个设施里的猕猴身上。实验接种结核分枝杆菌的猕猴。出现结核病(TB)的临床症状。新的体视学方法正被应用于在尸检时定量评估这些动物的肺叶的细菌负荷和病变(肉芽肿)。此外，在活跃期获得X射线照片和计算机断层扫描(CT)图像，用于非侵入性监测结核病疾病的进展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The animal biosafety level-3 (ABSL-3) facility at the California National Primate Research Center was completed and prepared for studies of Mycobacterium tuberculosis (M. tb.) in rhesus macaques. The ABSL-3 facility included both rooms for housing animals and a fully equipped necropsy suite for comprehensive pathologic analysis. Veterinary and research support staff were trained for studies of M. tb. in macaques in this facility. Macaques experimentally inoculated with M.tb. showed clinical signs of tuberculosis (TB). Novel stereology methods are being applied to quantitatively assess bacterial burden and lesions (granulomas) in lung lobes of these animals at necropsy. In addition, X-ray radiographs and computerized tomography (CT) images were obtained during the live-phase for non-invasive monitoring of tuberculosis disease progression.