Signal Processing And Instrumentation Section

信号处理和仪器科(SPIS)的研究和开发活动是与NIH研究所的科学家共同努力的，通常导致开发独特的、专门的生物医学仪器。其他项目涉及系统模拟和数据分析所需的信号和视频处理算法开发。SPIS的能力和成就使该集团成为NIH这类工程研究和技术开发的焦点。示范技术和方法开发项目以及相关的研究研究包括： 1.激光捕获组织显微切割(Lcm)技术用于正常发育和病理的大分子分析 2.细胞内分离鉴定细胞器蛋白质的表达组织显微切割(Xmd)方法 3.组织微阵列技术 4.双光子激发荧光显微镜(Tpefm)体内方法学 5.荧光光活化定位显微镜(Fpalm)纳米级成像 6.实现体内功能和生理成像的电子顺磁共振方法 7.基因芯片和蛋白质芯片技术 8.磁共振成像(Mri)和功能mri(Fmri)方法和设备 9.用于分析假定的诊断和治疗示踪剂的生物分布的伽马相机成像 10.用于疾病检测、监测和指导手术的荧光成像 11.组织定量表征的光学偏振成像和统计分析 12.设施笼内定量行为评估的自动老鼠活动监测系统(MAMS) 13.临床病理组织固定和切片方法 14.用于分子分析的微流体、微制造和微分析技术 15.单分子、dna和染色质纤维机械和操纵技术 16.促进健康和节律的时间和光谱可编程照明技术 17.使用基于mr的个性化模具将在体前列腺癌mri与组织病理学相关联。 18.色觉回路方法的苍蝇视动行为分析和遗传剖析 19.行为神经生理学仪器和方法的自主措施 20.用于纳米尺度分析细胞和组织的动态、构象和组织特征的振动光谱近场扫描显微镜成像 21.光动力疗法(PDT)在癌症治疗中的应用 22.语音采集、分析和实时自适应处理方法 23.非人灵长类母胎监测技术用于研究胎儿发育过程中的心理、生理和行为过程 24.生物力学实时测量与分析技术 25.肌纤维张力瞬变测试及分析技术 26.通过小鼠模型试验研究疼痛的遗传学和神经元特异性传递机制的自动刺激和监测仪器 27.细菌视紫红质能量转导机制的高速扫描光谱分析 28.实时多光谱内窥镜成像辅助手术 29.正电子发射断层扫描(PET)成像 30.共焦显微镜成像 31.染色体显微切割技术 32.无创实时在体红外成像技术评价血管内皮功能 33.原子力显微镜成像 34.磁共振弹性成像(MRE)成像 35.超声成像

The research and development activities of the Signal Processing and Instrumentation Section (SPIS) are collaborative efforts with NIH Institute scientists, and often result in the development of unique, specialized biomedical instruments. Other projects involve signal and video processing algorithm development required for system simulation and data analysis. SPIS capabilities and accomplishments have established the group as the focal point for this type of engineering research and technology development at the NIH. Example technology and methodology development projects, as well as associated research studies include: 1. laser capture tissue microdissection (LCM) technologies for macromolecular analysis of normal development and pathology 2. expression tissue microdissection (xMD) methodologies enabling subcellular isolation for identification of organelle proteins 3. tissue microarray (TMA) technologies 4. two-photon excitation fluorescence microscopy (TPEFM) in-vivo methodologies 5. fluorescence photo activation localization microscopy (FPALM) nanoscale imaging 6. electron paramagnetic resonance (EPR) methodologies enabling in vivo functional and physiological imaging 7. cDNA and protein microarray technologies 8. magnetic resonance imaging (MRI) and functional MRI (fMRI) methodologies and devices 9. gamma camera imaging for analyzing bio-distribution of putative diagnostic and therapeutic radiotracers 10. fluorescence imaging for disease detection, monitoring, and guided surgery 11. optical polarization imaging and statistical analysis for quantitative characterization of tissue 12. automated mouse activity monitoring system (MAMS) for quantitative behavioral assessment in facility cages 13. clinical pathology tissue fixation and sectioning methodologies 14. microfluidics, microfabrication, and microanalysis technologies for molecular analysis 15. single molecule, DNA, and chromatin fiber mechanics and manipulation technologies 16. temporal and spectral programmable lighting technologies for health and rhythm entrainment 17. correlating in vivo prostate MRI and histopathology using individualized MR-Based molds 18. fly optomotor behavioral analysis and genetic dissection of color-vision circuits methodologies 19. autonomic measures for behavioral neurophysiology instrumentation and methodologies 20. vibrational spectroscopic near-field scanning microscopy imaging for nanoscale analysis of dynamical, conformational and organizational characteristics of cells and tissues 21. photodynamic therapy (PDT) technologies for cancer treatment 22. speech acquisition, analysis, and real-time adaptive processing methodologies 23. nonhuman primate maternal-fetal monitoring technologies for investigation of psychological, physiological, and behavior processes during fetal development 24. biomechanics real-time measurement and analysis technologies 25. muscle fiber tension transient instrumentation and analysis technologies 26. automated stimulation and monitoring instrumentation for investigation of genetics and neuro-specific transmission mechanisms of pain via mouse model assays 27. high-speed scanning optical spectrometry for analysis of bacteriorhodopsin energy transduction mechanisms 28. real-time multispectral endoscope imaging as an aid to surgery 29. positron emission tomography (PET) imaging 30. confocal microscopy imaging 31. chromosome microdissection technologies 32. non-invasive real-time in-vivo infrared imaging for assessment of endothelial function 33. atomic force microscopy (AFM) imaging 34. magnetic resonance elastography (MRE) imaging 35. ultrasound imaging